phosphorylated mek Search Results


polyclonal antibodies against the phosphorylated forms of erks and mek  (Promega)
90
Promega polyclonal antibodies against the phosphorylated forms of erks and mek
Fig. 1. cAMP activates ERKs, <t>MEK</t> and B-Raf in melanocyte. Lysates from non-stimulated cells (NS), cells stimulated for 10 min with the cAMP-elevating agents, IBMX plus forskolin (I+F) or α-MSH (α-M) or with the protein kinase C activator, TPA (T) were subjected to western blot analysis with an anti-phospho-ERK antibody (A) or with an anti-phospho-MEK1 antibody (B). <t>A</t> <t>polyclonal</t> antibody to ERK2 (1/2000) (A) or MEK (1/1000) (B) was used as a control of protein loading. (C) B16 cells were treated as above or pre-incubated for 20 min with cAMP-elevating agents and then stimulated for 10 min with TPA (I+F+T). B-Raf or Raf-1 was then immunoprecipitated and their kinase activities were assayed using the His–MEK fusion protein as substrate. Samples were resolved by 10% SDS–PAGE and analysed by autoradiography.
Polyclonal Antibodies Against The Phosphorylated Forms Of Erks And Mek, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphorylated+mek/polyclonal+antibodies+against+the+phosphorylated+forms+of+erks+and+mek/pmc00203360-369-10-13
Average 90 stars, based on 1 article reviews
polyclonal antibodies against the phosphorylated forms of erks and mek - by Bioz Stars, 2026-10
90/100 stars
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phosphorylated mek  (Tsang MD Inc)
90
Tsang MD Inc phosphorylated mek
Fig. 1. cAMP activates ERKs, <t>MEK</t> and B-Raf in melanocyte. Lysates from non-stimulated cells (NS), cells stimulated for 10 min with the cAMP-elevating agents, IBMX plus forskolin (I+F) or α-MSH (α-M) or with the protein kinase C activator, TPA (T) were subjected to western blot analysis with an anti-phospho-ERK antibody (A) or with an anti-phospho-MEK1 antibody (B). <t>A</t> <t>polyclonal</t> antibody to ERK2 (1/2000) (A) or MEK (1/1000) (B) was used as a control of protein loading. (C) B16 cells were treated as above or pre-incubated for 20 min with cAMP-elevating agents and then stimulated for 10 min with TPA (I+F+T). B-Raf or Raf-1 was then immunoprecipitated and their kinase activities were assayed using the His–MEK fusion protein as substrate. Samples were resolved by 10% SDS–PAGE and analysed by autoradiography.
Phosphorylated Mek, supplied by Tsang MD Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphorylated+mek/phosphorylated+mek/pm15239952-79-12-24
Average 90 stars, based on 1 article reviews
phosphorylated mek - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
active phosphorylated mek  (Biaffin Inc)
90
Biaffin Inc active phosphorylated mek
Fig. 1. cAMP activates ERKs, <t>MEK</t> and B-Raf in melanocyte. Lysates from non-stimulated cells (NS), cells stimulated for 10 min with the cAMP-elevating agents, IBMX plus forskolin (I+F) or α-MSH (α-M) or with the protein kinase C activator, TPA (T) were subjected to western blot analysis with an anti-phospho-ERK antibody (A) or with an anti-phospho-MEK1 antibody (B). <t>A</t> <t>polyclonal</t> antibody to ERK2 (1/2000) (A) or MEK (1/1000) (B) was used as a control of protein loading. (C) B16 cells were treated as above or pre-incubated for 20 min with cAMP-elevating agents and then stimulated for 10 min with TPA (I+F+T). B-Raf or Raf-1 was then immunoprecipitated and their kinase activities were assayed using the His–MEK fusion protein as substrate. Samples were resolved by 10% SDS–PAGE and analysed by autoradiography.
Active Phosphorylated Mek, supplied by Biaffin Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphorylated+mek/active+phosphorylated+mek/pm24507826-112-7-9
Average 90 stars, based on 1 article reviews
active phosphorylated mek - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


Fig. 1. cAMP activates ERKs, MEK and B-Raf in melanocyte. Lysates from non-stimulated cells (NS), cells stimulated for 10 min with the cAMP-elevating agents, IBMX plus forskolin (I+F) or α-MSH (α-M) or with the protein kinase C activator, TPA (T) were subjected to western blot analysis with an anti-phospho-ERK antibody (A) or with an anti-phospho-MEK1 antibody (B). A polyclonal antibody to ERK2 (1/2000) (A) or MEK (1/1000) (B) was used as a control of protein loading. (C) B16 cells were treated as above or pre-incubated for 20 min with cAMP-elevating agents and then stimulated for 10 min with TPA (I+F+T). B-Raf or Raf-1 was then immunoprecipitated and their kinase activities were assayed using the His–MEK fusion protein as substrate. Samples were resolved by 10% SDS–PAGE and analysed by autoradiography.

Journal:

Article Title: Ras mediates the cAMP-dependent activation of extracellular signal-regulated kinases (ERKs) in melanocytes

doi: 10.1093/emboj/19.12.2900

Figure Lengend Snippet: Fig. 1. cAMP activates ERKs, MEK and B-Raf in melanocyte. Lysates from non-stimulated cells (NS), cells stimulated for 10 min with the cAMP-elevating agents, IBMX plus forskolin (I+F) or α-MSH (α-M) or with the protein kinase C activator, TPA (T) were subjected to western blot analysis with an anti-phospho-ERK antibody (A) or with an anti-phospho-MEK1 antibody (B). A polyclonal antibody to ERK2 (1/2000) (A) or MEK (1/1000) (B) was used as a control of protein loading. (C) B16 cells were treated as above or pre-incubated for 20 min with cAMP-elevating agents and then stimulated for 10 min with TPA (I+F+T). B-Raf or Raf-1 was then immunoprecipitated and their kinase activities were assayed using the His–MEK fusion protein as substrate. Samples were resolved by 10% SDS–PAGE and analysed by autoradiography.

Article Snippet: The polyclonal antibodies against the phosphorylated forms of ERKs and MEK were from Promega France.

Techniques: Western Blot, Control, Incubation, Immunoprecipitation, SDS Page, Autoradiography

Fig. 9. Model for the cAMP-dependent activation of MAPK in melanocyte cells. α-MSH binds the melanocyte receptor, MC1-R, and activates adenylyl cyclase (AC), leading to an increase in the intracellular cAMP content. cAMP activates Rap-1 through phosphorylation by PKA via cAMP-dependent Rap-1 GEFs (Epac/cAMP Rap-1 GEFs). In melanocytes, cAMP could also directly activate an unidentified cell-specific cAMP-dependent Ras GEF (cAMP Ras GEF) which would activate Ras in response to cAMP. Ras activates Raf-1 and B-Raf, but Raf-1 is inhibited by an unknown mechanism, so activated B-Raf mediates the Ras activation of MEK and ERKs.

Journal:

Article Title: Ras mediates the cAMP-dependent activation of extracellular signal-regulated kinases (ERKs) in melanocytes

doi: 10.1093/emboj/19.12.2900

Figure Lengend Snippet: Fig. 9. Model for the cAMP-dependent activation of MAPK in melanocyte cells. α-MSH binds the melanocyte receptor, MC1-R, and activates adenylyl cyclase (AC), leading to an increase in the intracellular cAMP content. cAMP activates Rap-1 through phosphorylation by PKA via cAMP-dependent Rap-1 GEFs (Epac/cAMP Rap-1 GEFs). In melanocytes, cAMP could also directly activate an unidentified cell-specific cAMP-dependent Ras GEF (cAMP Ras GEF) which would activate Ras in response to cAMP. Ras activates Raf-1 and B-Raf, but Raf-1 is inhibited by an unknown mechanism, so activated B-Raf mediates the Ras activation of MEK and ERKs.

Article Snippet: The polyclonal antibodies against the phosphorylated forms of ERKs and MEK were from Promega France.

Techniques: Activation Assay, Phospho-proteomics